Dijagnostičke vrijednosti proteina akutne faze u iranskoga domaćega goveda invadiranoga praživotinjom Theileria annulata

This study was conducted to assess the pattern of changes and the relative value of acute phase proteins (APP) including haptoglobin (Hp), serum amyloid A (SAA), ceruloplasmin and fi brinogen in Iranian indigenous cattle infected with Theileria annulata. The diseased group comprised 24 Iranian indigenous dairy cattle, 2-3 years old, naturally infected with Theileria annulata. The infected animals were divided into 4 subgroups with different parasitemia rates (<1% and 1-3%). As a control group, 10 uninfected cattle were also sampled. Blood samples were collected and all measurements were made using validated methods. There were significant differences in red blood cells (RBCs), packed cell volume (PCV), hemoglobin (Hb) and concentrations of Hp, SAA, ceruloplasmin and fibrinogen between healthy cattle and those infected with T. annulata with different parasitemia rates (P<0.05). As the parasitemia rate increased in infected cattle, a signifi cant decrease was observed in RBCs, PCV and Hb. In contrast, with the increase in the parasitemia rate, a significant increase in Hp, SAA, ceruloplasmin and fibrinogen was evident. The optimal cut-off point was set by the receiver operating characteristics (ROC) method to >5.68 μg/mL for SAA, >0.09 g/L for Hp, >0.049 g/L for ceruloplasmin and >1.90 g/L for fibrinogen, with corresponding 71.50% sensitivity and 100% specificity for SAA, 83.30% sensitivity and 70% specificity for Hp, 50% sensitivity and 90% specificity for ceruloplasmin and 71.30% sensitivity and 80% specificity for fi brinogen. In conclusion, measuring SAA with the highest sensitivity, specificity and AUC compared to other APPs, can be a suitable indicator of inflammatory reactions in indigenous cattle infected with Theileria annulata.Istraživanje je provedeno s potrebom da se utvrdi dinamika promjena i relativne vrijednosti proteina akutne faze, uključujući haptoglobin (Hp), serumski amiloid A, ceruloplazmin i fibrinogen, u iranskoga domaćega goveda invadiranoga praživotinjom Theileria annulata. Skupina pokusnih životinja sadržavala je ukupno 24 iranska domaća mliječna goveda u dobi od dvije do tri godine invadirana praživotinjom Theileria annulata. Invadirane životinje bile su podijeljene u četiri podskupine s obzirom na različite razine parazitemije (5,68 μg/mL za serumski amiloid A, >0,09 za haptoglobin, >0,049 g/L za ceruloplazmin i >1,90 g/L za fibrinogen. Za serumski je amiloid osjetljivost iznosila 71,50% dok je specifičnost bila 100%. Osjetljivost je za haptoglobin iznosila 83,30% dok je specifičnost iznosila 70%. Za ceruloplazmin je osjetljivost iznosila 50%, a specifičnost 90%. Za fibrinogen je osjetljivost iznosila 71,30% dok je specifičnost iznosila 80%. Zaključno se može reći da mjerenje vrijednosti serumskoga amiloida A može biti prikladan pokazatelj upale uzrokovane praživotinjom Theileria annulata jer se u odnosu na ostale proteine akutne faze odlikuje najvišom razinom osjetljivosti, specifičnosti i AUC